murAA [2019-07-04 12:40:31]

UDP-N-acetylglucosamine 1-carboxyvinyltransferase

locus: BSU36760

pI: 5.45

mw: 46.54 kDa

protein length: 436 aa

gene length: 1311 bp

function: peptidoglycan precursor biosynthesis

product: UDP-N-acetylglucosamine 1-carboxyvinyltransferase

essential: yes

ec: 2.5.1.7

synonyms: murA

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.1|Biosynthesis of peptidoglycan]
• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.1|Biosynthesis of cell wall components] → [category|SW 2.6.1.1|Biosynthesis of peptidoglycan]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

Gene

Coordinates: 3,777,949 → 3,779,259

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• Phosphoenolpyruvate UDP-N-acetyl-D-glucosamine = phosphate UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine (according to Swiss-Prot)

Protein family

• MurA subfamily (according to Swiss-Prot)

Paralogous protein(s)

• [protein|523B01B5E7141C7DFC28CE67EB92580F49C20844|MurAB]

Structure

• [PDB|3SG1] (from ''B. anthracis'', 79% identity, 94% similarity)

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Additional information

• [protein|DA763F7C2192655C590752C2A285ADF902B11BCA|MurAA] is subject to [protein|86A2F2F65290F4471D6FD03B694821C66C180D8A|ClpC]-[protein|CB06A70DE7462CEB7AF5D8C28943C878DD56DE1A|ClpP]-dependent degradation upon glucose starvation [Pubmed|14763982,17981983]

Expression and Regulation

Operons

• genes: [gene|32CE4F77F08F926152BECAB56E2841E1FFB27600|ywzB]-[gene|3A8E0417C4EC85FDDE1178B775F6579C8F1009C6|spoIIT]-[gene|DA763F7C2192655C590752C2A285ADF902B11BCA|murAA]
  description: [Pubmed|22383849]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|22383849], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]: sigma factor, [Pubmed|26735940], in [regulon|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF regulon]

  regulation

  • expressed early during sporulation in the forespore ([protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]) [Pubmed|26735940]

• genes: [gene|DA763F7C2192655C590752C2A285ADF902B11BCA|murAA]
  description: [Pubmed|9353933]

  regulation

  • expressed early during sporulation in the forespore ([protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]) [Pubmed|26735940]

• genes: [gene|3A8E0417C4EC85FDDE1178B775F6579C8F1009C6|spoIIT]-[gene|DA763F7C2192655C590752C2A285ADF902B11BCA|murAA]
  description: [Pubmed|26735940]

  regulation

  • expressed early during sporulation in the forespore ([protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]) [Pubmed|26735940]

additional information

• the mRNA is cleaved by [protein|BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981|RNase III] [PubMed|26883633]

Biological materials

Expression vectors

• pRSETA-murAA, Expression of His(6)-MurAA, avalable in [SW|Ulf Gerth]'s lab [Pubmed|14763982]
• pGP2597: (IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172]), available in [SW|Jörg Stülke]'s lab

Antibody

• avalable in [SW|Ulf Gerth]'s lab [Pubmed|14763982]

References

Reviews

• 21822642

Original publications

• 14763982,18063720,19899805,14763982,8994972,9485407,26735940,22383849,26883633